JAK2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1/64,000 for detection of the JAK2 peptide coated on ELISA plate. Western blot: 1/2,000 to detect 130kDa JAK2 in TF-1 cell extract.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide derived from the human JAK2 peptide, conjugated with KLH
Specificity	This antibody reacts with the 130kDa JAK2 on human and other species with consensus sequence.
Formulation	0.01M PBS, pH 7.4 State: Azide Free State: Lyophilized Ig fraction
Reconstitution Method	Restore with double distilled water to adjust the final concentration to 1.00 mg/ml.
Purification	Protein G affinity
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	Janus kinase 2
Database Link	Entrez Gene 3717 Human UniProt ID O60674
Background	Janus kinase 2 (JAK2) is a non-receptor tyrosine kinase that plays an important role in signal transduction through its association with receptors on the cell membrane. When these receptors are activated by extracellular signals, the receptors activate JAK2 which in turn phosphorylates the tyrosine residues on the receptors. The phosphorylation enables the binding of downstream signaling molecules, primarily, signal transducer and activator of transcription (STAT) proteins. STATs possess a SHdomain that specifically binds to phosphorylated tyrosine residue on the receptor. STAT in cytoplasma is activated after binding and migrates to nucleus to activate gene transcription. JAK2 mediates the transduction of a variety of extracellular signals in this pattern. The receptors that are associated with JAK2 include type II cytokine receptors, GM-CSF receptor family, gp130 receptor family, growth hormone receptor, prolactin receptor and EPO receptor.
Synonyms	Janus kinase 2, JAK-2
Reference Data

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