KRIT1 Rabbit Polyclonal Antibody

CAT#: AP26021PU-N

KRIT1 rabbit polyclonal antibody, Purified



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CNY 3,450.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Applications IF, WB
Recommend Dilution Western Blot: 1-5 μg/ml.
Immunofluorescence: 1-10 μg/ml.
Immunohistochemistry: 1/200.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>95%) recombinant Human CCM-1 (Met1-Ser736) derived from E. coli fused to a C-teminal His-tag (6 x His) (Cat.-No AR26002PU-N).
Specificity This antibody is anti-His depleated.
It detects KRIT1 / CCM1.
Formulation 5mM PBS, pH 7.2
State: Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Centrifuge vial prior to opening.
Purification Protein A Chromatography
Conjugation Unconjugated
Storage Condition

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Gene Name KRIT1, ankyrin repeat containing
Background Cerebral Cavernous Malformations (CCM) are frequent vascular abnormalities caused by mutations in one of the CCM genes. CCM-1 (also known as KRIT1) stabilizes endothelial junctions and is essential for vascular morphogenesis in mouse embryos. However, cellular functions of CCM-1 during the early steps of the CCM pathogenesis remain unknown. It was shown that CCM-1 represents an antiangiogenic protein to keep the human endothelium quiescent. CCM-1 inhibits endothelial proliferation, apoptosis, migration, lumen formation, and sprouting angiogenesis in primary human endothelial cells. CCM-1 strongly induces DLL4-NOTCH signaling, which promotes AKT phosphorylation but reduces phosphorylation of the mitogen-activated protein kinase ERK. Consistently, blocking of NOTCH activity alleviates CCM-1 effects. ERK phosphorylation is increased in human CCM lesions. Transplantation of CCM-1-silenced human endothelial cells into SCID mice recapitulates hallmarks of the CCM pathology and serves as a unique CCM model system.
Synonyms Krev interaction trapped 1
Reference Data
Protein Families Druggable Genome
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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