Il5 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Sandwich ELISA: To detect Mouse IL-5 by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Purified Anti-Mouse IL5 (AP23789PU-N or AP23789PU-S) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IL-5. Direct ELISA: To detect Mouse IL-5 by Direct ELISA (using 100μl/well antibody solution) a concentration of ~1.0 μg/ml of this antibody is required. This Biotin conjugated antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IL-5. Western Blot: To detect Mouse Il-5by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse IL-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure recombinant Murine IL-5
Specificity	Recognizes Interleukin-5/IL5.
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing an immobilized Murine IL-5 matrix
Conjugation	Biotin
Storage Condition	Store the lyophilized antibody at -20°C. Following reconstitution it is stable for two weeks at 2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C. Avoid repeated freezing and thawing.
Gene Name	interleukin 5
Database Link	Entrez Gene 16191 Mouse UniProt ID P04401
Background	Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and Mouse IL5 have 70% amino acid sequence homology.
Synonyms	IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF
Reference Data

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