PMVK Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: Use at 0.1-0.5 μg/ml with the appropriate system to detect PMVK in cells and tissues. Imunohistochemistry on Paraffin Sections: Use at 0.5-1 μg/ml to detect PMVK in formalin fixed and paraffin embedded tissues.  Heat Antigen Retrieval isn recommended.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a sequence mapping at the middle region of Human PMVK
Specificity	This antibody detects PMVK (mid region). No cross reactivity with other proteins.
Formulation	0.2 mg Na2HPO4, 0.9 mg NaCl State: Aff - Purified State: Lyophilized purified Ig fraction Stabilizer: 5 mg BSA Preservative: 0.05 mg Thimerosal, 0.05 mg Sodium Azide
Reconstitution Method	0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	phosphomevalonate kinase
Database Link	Entrez Gene 68603 MouseEntrez Gene 10654 Human UniProt ID Q15126
Background	PMVK (phosphomevalonate kinase) is a cytosolic enzyme that catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate. It is mapped to chromosome 1p13-1q22-23 and spans more than 8.4 kb in the human genome. PMVK is a peroxisomal protein which requires the C-terminal peroxisomal targeting signal, Ser-Arg-Leu, for localization to the organelle. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. And PMKase gene expression is subject to regulation by sterol at the level of transcription. It is a single copy gene covering less than 15 kb in the human genome. The human PMKase amino acid sequence contains a consensus peroxisomal targeting sequence (PTS-1), Ser-Arg-Leu, at the C terminus of the protein.
Synonyms	Phosphomevalonate kinase, PMKase, PMKI, HUMPMKI
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Terpenoid backbone biosynthesis

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