GJB2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	Western blot: At 1-2μg/ml with the appropriate system to detect Connexin 26 in cells and tissues. Immunocytochemistry.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide mapping at the middle region of rat Connexin 26
Specificity	This antibody detects GJB2 / Cx26 (mid region). No cross reactivity with other proteins.
Formulation	50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	gap junction protein beta 2
Database Link	Entrez Gene 14619 MouseEntrez Gene 394266 RatEntrez Gene 2706 Human UniProt ID P29033
Background	Connexin26(CX26), also known as GAP junction protein, beta2, GJB2. Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins,called connexins, purified from fractions of enriched gap junctions from different tissues differ. The 3-prime untranslated region of the CX26 transcript contains a putative mRNA instability sequence. The deduced 226-amino acid protein has a calculated molecular mass of about 26 kD. CX26 shares 92.5% identity with rat Cx26. connexin 26 (GJB2) is assigned to human chromosome 13q11-q12. Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. Connexin 26 gene (GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation.
Synonyms	Gap junction beta-2 protein, Connexin-26
Reference Data
Protein Families	Druggable Genome, Ion Channels: Other, Transmembrane

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