GJA1 (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: At 2µg/ml with the appropriate system to detect Connexin 43 in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2µg/ml to detect Connexin 43 in formalin fixed and paraffin embedded tissues. Immunocytochemistry.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide mapping at the C-terminus of human Connexin 43
Specificity	This antibody detects GJA1 / Cx43 at C-term. No cross reactivity with other proteins.
Formulation	50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	gap junction protein alpha 1
Database Link	Entrez Gene 14609 MouseEntrez Gene 24392 RatEntrez Gene 2697 Human UniProt ID P17302
Background	Connexins 43 (Cx43), also called GAP Junction Protein, alpha-1(GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene,which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.
Synonyms	Gap junction alpha-1 protein, Connexin-43, GJAL
Reference Data
Protein Families	Druggable Genome, Ion Channels: Other, Transmembrane
Protein Pathways	Arrhythmogenic right ventricular cardiomyopathy (ARVC), Gap junction

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