DNA Polymerase gamma (POLG) (829-1201) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunocytochemistry: 1/100 - 1/200. Immunofluorescence: 1/100 - 1/200. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Western Blot: 1/1000 - 1/10000.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein fragment containing a sequence corresponding to a region within amino acids 829 and 1201 of Human DNA polymerase gamma
Specificity	This antibody detects DNA polymerase gamma at aa 829-1201.
Formulation	PBS, pH 7 containing 1% BSA, 20% Glycerol and 0.01% Thimerosal State: Aff - Purified State: Liquid purified Ig fraciton
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	polymerase (DNA) gamma, catalytic subunit
Database Link	Entrez Gene 18975 MouseEntrez Gene 85472 RatEntrez Gene 5428 Human UniProt ID P54098
Background	As the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil DNA glycosylase and apurinic / apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic / apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.
Synonyms	POLG, MDP1, POLGA, DNA polymerase subunit gamma-1, PolG-alpha
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways

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