VPS29 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: Limit Dlution: 1/16000. Western Blot: Preliminary experiments gave an approx 60kDa band in Human Spleen lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 20.5kDa according to NP_057310.1 and 20.9kDa according to NP_476528.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry on Paraffin Sections: 4-8 µg/ml. In paraffin embedded Human Uterus shows vesicular and membranous staining in the endometrial glands.
Reactivity	Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_057310.1; NP_476528.1.
Specificity	This antibody is expected to recognize both reported isoforms (NP_057310.1 and NP_476528.1).
Formulation	Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	VPS29 retromer complex component
Database Link	Entrez Gene 56433 MouseEntrez Gene 288666 RatEntrez Gene 51699 Human UniProt ID Q9UBQ0
Background	VPS29, VPS30, and VPS35 proteins are required for the normal recycling of Vps10p, a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi.
Synonyms	Vesicle protein sorting 29, PEP11, DC7, DC15, MDS007
Reference Data

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