Properdin (CFP) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ID, IP
Recommend Dilution	Can be used in precipitating techniques as Immunoelectrophoresis and single or Double Radial Immunodiffusion. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Recommended Dilutions: Immunoelectrophoresis: Use 2 µl human plasma or equivalent against 120 µl antiserum. Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. Single Radial Immunodiffusion: Use 1% antiserum in the gel. Antibody Titre: Precipitin titre 1/8 when tested against normal Human Serum in agar-block immunodiffusion titration.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	The antigen has been isolated from pooled Human plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The defined antibody specificity is restricted to Properdin as tested at the level of sensitivity of immunoprecipitation techniques. A single precipitin line is obtained in immunoelectrophoresis, bidimensional electrophoresis and double radial immunodiffusion (Ouchterlony) against normal plasma, which shows a reaction of identity with the precipitated purified properdin. No reaction is obtained with any other plasma protein. The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Formulation	0.2M Tris, 0.5M NaCl, pH7.6 State: Serum State: Liquid delipidated, defibrinated and 0.2µm filtered serum Preservative: 0.09% Sodium Azide
Concentration	Total protein and IgG concentrations in the antiserum is approximatly 20 mg/ml.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	complement factor properdin
Database Link	Entrez Gene 5199 Human UniProt ID P27918
Background	Properdin is a factor of the alternative complement activation pathway. It has a molecular weight of 222,000 and consists of four peptide chains. Properdin is stabilizing the C3 convertase C3bBb. The mean concentration in plasma is 20 mg/ml.
Synonyms	CFP, PFC, Complement factor P
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the anti­serum.
Reference Data
Protein Families	Secreted Protein

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