Properdin (CFP) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IHC, IP
Recommend Dilution	Can be used as reagent for the direct detection of Properdin in Human cells, tissues and body fluids in Immunofluorescence, as detection reagent in non-isotopic methodology and Solid Phase Immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: 1/10-1/60.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	The antigen has been isolated from pooled Human plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	In Immunoelectrophoresis against Human serum a single precipitin line is obtained. The antiserum does not react with any other protein component of Human serum or plasma. The antiserum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Formulation	PBS, pH 7.2 without preservatives Label: FITC State: Lyophilized purified IgG fraction Label: Fluorescein Isothiocyanate isomer 1 Absorption emission: 492 nm / 515 nm Molar radio: Fluorescein/IgG ~1.4
Reconstitution Method	Restore by adding 1 ml of sterile distilled water
Concentration	lot specific
Purification	Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt Precipitation and purification of the IgG fraction by DEAE-Chromatography
Conjugation	FITC
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	complement factor properdin
Database Link	Entrez Gene 5199 Human UniProt ID P27918
Background	Properdin is a factor of the alternative complement activation pathway. It has a molecular weight of 222,000 and consists of four peptide chains. Properdin is stabilizing the C3 convertase C3bBb. The mean concentration in plasma is 20 mg/ml.
Synonyms	CFP, PFC, Complement factor P
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other dog serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Reference Data
Protein Families	Secreted Protein

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