poxB Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Working Dilutions: Non-precipitating antibody-binding techniques: 1/1,000-1/8,000.
Reactivity	Bacteria
Host	Rabbit
Clonality	Polyclonal
Immunogen	Pyruvate oxidase isolated and purified from bacteria. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized hyperimmune IgG fraction
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated freezing and thawing.
Database Link	UniProt ID P07003
Background	Catalyzes the oxidation of pyruvate to acetylphosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.
Synonyms	Pyruvate dehydrogenase ubiquinone, POX
Reference Data

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