PHKB Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques). Working dilutions: vary widely, but may be between 1/200 and 1/2000.
Reactivity	Rabbit
Host	Sheep
Clonality	Polyclonal
Immunogen	Phosphorylase b isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	Biotin-conjugated IgG fraction of polyclonal sheep antiserum to phosphorylase b from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized Hyperimmune IgG fraction Molar radio: Biotin/IgG ~5.8
Reconstitution Method	Restore with 1,0 ml distilled water
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Biotin
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 100009137 Rabbit UniProt ID P12798
Synonyms	DKFZp781E15103; FLJ41698
Reference Data

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