elF2 alpha (EIF2S1) pSer51 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/50 - 1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of eIF2alpha protein only when phosphorylated at Ser51.
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide
Concentration	1.0 mg/ml
Purification	Affinity chromatography (> 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 38 kDa
Gene Name	eukaryotic translation initiation factor 2 subunit alpha
Database Link	Entrez Gene 13665 MouseEntrez Gene 54318 RatEntrez Gene 1965 Human UniProt ID P05198
Background	Phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2alpha) is known to be an important translational control mechanism. Regulation of mammalian eIF2alpha activity is directly governed by specific phosphorylation on Serine 52. In mammalian cells, eIF2alpha is phosphorylated at Serine 52 by at least two kinases, the haem-controlled repressor and the interferon-inducible double stranded RNA-dependent protein kinase. This phosphorylation event results in an inhibition in the exchange of bound nucleotides and the recycling of eIF2alpha. In addition, phosphorylation of eIF2alpha blocks the GDP-GTP exchange activity of eIF2beta, resulting in the suppression of protein synthesis.
Synonyms	EIF2-A, eIF-2A, eIF-2-alpha, eIF-2alpha, eIF2 alpha
Reference Data

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