EIF4E pSer209 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/50 - 1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of eIF4E protein only when phosphorylated at Ser209.
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide
Concentration	1.0 mg/ml
Purification	Affinity chromatography (> 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 25 kDa
Gene Name	eukaryotic translation initiation factor 4E
Database Link	Entrez Gene 13684 MouseEntrez Gene 117045 RatEntrez Gene 1977 Human UniProt ID P06730
Background	The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E is a 25 kDa protein that binds to the mRNA “cap” during an early step in the initiation of protein synthesis. eIF4A is a 46 kDa protein that acts as an ATP-dependent RNA helicase. eIF4G is 154 kDa protein that acts as a bridge between eIF4E, eIF4A, and the eIF3 complex.
Synonyms	eIF-4F 25 kDa subunit, EIF-4E, EIF4EL1, EIF4F
Reference Data
Protein Pathways	Insulin signaling pathway, mTOR signaling pathway

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