Pepsinogen II (PGC) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/50 - 1/200.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of Pepsin C protein. (region surrounding Phe306)
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% sodium azide
Concentration	1.0 mg/ml
Purification	Affinity chromatography (> 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 42 kDa
Gene Name	progastricsin (pepsinogen C)
Database Link	Entrez Gene 5225 Human UniProt ID P20142
Background	Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, Pepsinogen, by removal of a 41 amino acid segment from the amino end. Pepsinogen is synthesized in the stomach lining; hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for Pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. It shows extremely broad specificity, and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The amino acid composition of Pepsin C differs from those of Pepsinogen and Pepsin, especially in the content of basic amino acids, glutamic acid, aspartic acid, leucine and isoleucine.
Synonyms	Pepsinogen C, Progastricsin, Gastricsin, PGC
Reference Data
Protein Families	Protease, Secreted Protein

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