GUCY1A1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 400-450 of Human GCS-α-1.
Specificity	This antibody detects endogenous levels of GCS-alpha-1 protein. (region surrounding Asp423)
Formulation	Phosphate buffered saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity-chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 77 kDa
Gene Name	guanylate cyclase 1, soluble, alpha 3
Database Link	Entrez Gene 2982 Human UniProt ID Q02108
Background	Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase, a soluble form (GCS or sGC), which act as receptors for nitric oxide and a membrane-bound receptor form (GC), which are peptide hormone receptors. The GC-C protein is composed of an extracellular domain, a single transmembrane domain, and a cytoplasmic region consisting of a kinase-like domain and a catalytic domain. It is expressed as two differentially glycosylated forms, a 130 kDa precursor form present in the endoplasmic reticulum and a 145 kDa form present on the plasma membrane. Ligand binding to the extracellular domain of GC-C promotes the accumulation of cGMP. GC-C acts as the receptor for heatstable enterotoxins, small peptides secreted by some pathogenic strains of E. coli that cause severe secretory diarrhea. GC-C also binds to guanylin and uroguanylin peptides, which modulate renal function in response to oral salt load.
Synonyms	GCS-alpha-3, GCS-alpha-1, GUC1A3, GUCSA3, GUCY1A1, sGC alpha
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction

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