Oxytocin neurophysin 1 (OXT) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of Neurophysin I protein. (region surrounding Lys40)
Formulation	Phosphate buffered saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~20 kDa
Gene Name	oxytocin/neurophysin I prepropeptide
Database Link	Entrez Gene 5020 Human UniProt ID P01178
Background	The nonapeptide hormones Arginine Vasopressin (ARGVasopressin or AVP) and Oxytocin are synthesized in the supraoptic and paraventricular nuclei of the hypothalamus together with their respective ”carrier” proteins, the neurophysins. Vasopressin and Oxytocin are produced by separate populations of magnocellular neurons in both nuclei. Neurophysin I (NPI) and Neurophysin II (NPII) function as carrier proteins for Oxytocin and Vasopressin, respectively. Oxytocin is a pituitary hormone which induces uterine contractions during childbirth and the ejection of milk from the mammary glands during nursing. Vasopressin is involved in the metabolism of water and electrolytes and has been identified as a vasoconstrictor. Both Neurophysin genes exist as three exons, with each exon encoding a functional protein domain. Studies show that the identically conserved middle region (exon B) is involved in NP-NP homodimer formation as well as being the site for the Glycine 17 to Valine point mutation responsible for familial diabetes insipidus. The genes encoding Neurophysin I and II map to human chromosome 20p13.
Synonyms	OXT, OT, OT-NPI, Neurophysin 1
Reference Data
Protein Families	Secreted Protein

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