Aromatase (CYP19A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000 Immunofluorescence: 1/50-1/200 Immunohistochemistry on Paraffin Sections: 1/50-1/200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of CYP19A1 protein. (region surrounding Lys243)
Formulation	Phosphate buffered saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 58 kDa
Gene Name	cytochrome P450 family 19 subfamily A member 1
Database Link	Entrez Gene 13075 MouseEntrez Gene 25147 RatEntrez Gene 1588 Human UniProt ID P11511
Background	P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. P450 enzymes can be classified, based on their sequence similarities, into distinct subfamilies, which include CYP1A and CYP2A. Other P450 family members include CYP19, also designated aromatase (P450arom), which catalyzes the conversion of C19 steroids to estrogens in various tissues, including placent,a gonads, adipose tissue, skin and brain. CYP19 expression is controlled by hormonally regulated promoters in different tissues and increased aromatase activity is associated with familial gynecomastia. Also, a polymorphic allele of CYP19 (repeat (TTTA)12) is present in a majority of breast cancer patients. P450 cholesterol 7α-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D 25-hydroxylation and is localized to the mitochondria in kidney and liver.
Synonyms	Cytochrome P450 19A1, CYPXIX, Estrogen synthetase, P450AROM, CYP19A1, ARO1, CYAR, CYP19
Reference Data
Protein Families	Druggable Genome, P450
Protein Pathways	Androgen and estrogen metabolism, Metabolic pathways

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