NCE2 (UBE2F) (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western blot: 0.05-0.2 µg/ml. Approx. 20kDa band observed in Mouse Liver lysates. Immunohistochemistry on Paraffin Sections: 2.5-3.8 µg/ml. In paraffin embedded Human Placenta shows membrane staining in trophoblasts.
Reactivity	Bovine, Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the C Terminus of the protein sequence according to NP_542409.1.
Specificity	This antibody recognizes NCE2 / UBE2F.
Formulation	Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ubiquitin conjugating enzyme E2 F (putative)
Database Link	Entrez Gene 140739 Human UniProt ID Q969M7
Background	NCE2 is a ubiquitin conjugating enzyme. Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.
Synonyms	NEDD8-conjugating enzyme 2, NEDD8 carrier protein, NEDD8 protein ligase UBE2F
Note	Calculated Molecular Weight: 21.1kDa (Human NP_542409.1 and Mouse NP_080730.1).
Reference Data

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