CCKAR Goat Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Peptide ELISA: Detection limit: 1/64000. Western blot: 0.1-0.3 µg/ml. Approx. 75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1). Data kindly provided by Dr. Morisset, Sherbrooke, Canada. Data accepted by International Journal of Endocrinology (in press). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022).
Reactivity	Mouse, Rabbit, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide from the internal region of the protein sequence according to NP_033957.1.
Specificity	This antibody detects CCK-A Receptor.
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide, with 0.5% BSA State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cholecystokinin A receptor
Database Link	UniProt ID P32238
Background	The cholecystokinin (CCK) family of peptide hormones have been implicated in numerous important physiologic events. These appear to be mediated through 2 general classes of receptors, A (CCKAR)and B (CCKBR), based on their binding affinities for CCK/gastrin family peptides. Through binding to class A receptors, CCK is a major physiologic mediator of gallbladder contraction and pancreatic enzyme secretion. It appears to play a role in slowing gastric emptying, relaxation of the sphincter of Oddi, and potentiation of insulin secretion. Further, it has been implicated as a mediator of pancreatic growth and tumorigenesis. Class A receptors have also been described in the anterior pituitary, myenteric plexus, and regions of the central nervous system, where they have been implicated in the pathogenesis of feeding disorders, Parkinson disease, schizophrenia, and drug addiction.
Synonyms	CCKA Receptor, Cholecystokinin receptor type A, Cholecystokinin-1 receptor, CCKAR, CCKRA
Reference Data

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