UBE2C (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, IP, WB
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western blot: 1-3 µg/ml. Detects a band of ~20KDa in mitotic (i.e Nocodazole arrested) HeLa whole cell lysates.  Flow Cytometry analysis of HeLa cells: 10 µg/ml. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads. Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide from C Terminus of the protein sequence according to NP_008950.1; NP_861515.1; NP_861516.1; NP_861517.1; NP_861518.1.
Specificity	This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
Formulation	Tris saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Gene Name	ubiquitin conjugating enzyme E2 C
Database Link	Entrez Gene 11065 Human UniProt ID O00762
Background	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression.
Synonyms	Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C, Ubiquitin carrier protein C
Reference Data

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