p21 (CDKN1A) pThr57 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	ELISA: 1/1,000. Dot Blot: 1/500. Immunohistochemistry: 1/50~1/100.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T57 of human P21CIP1.
Specificity	This antibody detects p21Cip1 pThr57.
Formulation	PBS with 0.09% (W/V) Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cyclin-dependent kinase inhibitor 1A
Database Link	Entrez Gene 1026 Human UniProt ID P38936
Background	This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
Synonyms	CAP20, CDKN1, CIP1, MDA6, MDA-6, PIC1, SDI1, WAF1
Note	Molecular weight: 17988 Da
Reference Data

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