Fadd pSer191 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S191 of mouse FADD.
Specificity	This antibody detects FADD pSer191.
Formulation	PBS with 0.09% (W/V) Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Fas (TNFRSF6)-associated via death domain
Database Link	Entrez Gene 14082 Mouse UniProt ID Q61160
Background	FADD is an apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.
Synonyms	FAS-associated death domain protein, MORT1, GIG3
Note	Molecular weight: 22960 Da
Reference Data

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