AKT1 pSer129 Rabbit Polyclonal Antibody
CAT#: AP12558PU-N
AKT1 pSer129 rabbit polyclonal antibody, Aff - Purified
Need it in bulk or conjugated?
Get a free quote
CNY 6,446.00
货期*
5周
规格
Specifications
Product Data | |
Applications | IHC, WB |
Recommend Dilution | ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser129 of human AKT1. |
Specificity | This antibody detects AKT1/PKB pSer129. Predicted to cross react with Mouse (100% Antigen Homology). |
Formulation | PBS with 0.09% (W/V) Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction. |
Concentration | lot specific |
Purification | Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Conjugation | Unconjugated |
Storage Condition | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | AKT serine/threonine kinase 1 |
Database Link | |
Background | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. |
Synonyms | Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT |
Note | Molecular weight: 55686 Da |
Reference Data |
Documents
Product Manuals |
FAQs |
SDS |
Resources
抗体相关资料 |
Customer
Reviews
Loading...