MASPIN (SERPINB5) Rabbit Polyclonal Antibody
CAT#: AP09877PU-S
MASPIN (SERPINB5) rabbit polyclonal antibody, Aff - Purified
Size: 100 ug
Conjugation: Biotin
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CNY 3,910.00
Specifications
Product Data | |
Applications | ELISA, IHC, WB |
Recommend Dilution | Sandwich ELISA: To detect Human Maspin by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Maspin (Cat.-No AP09877BT-N or AP09877BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Maspin. Western Blot: To detect Human Maspin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Maspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Paraffin Sections: This antibody stained formalin-fixed paraffin-embedded sections of human normal breast and human breast lobular carcinoma in situ. The recommended concentration is 7.8-15.625 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly purified E.coli derived 42.1 kDa recombinant Human Maspin (Cat.-No AR31112PU) |
Specificity | This antibody recognizes Maspin/SERPINB5. |
Formulation | PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction. |
Reconstitution Method | Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity Chromatography employing an immobilized Human Maspin matrix. |
Conjugation | Unconjugated |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Gene Name | serpin family B member 5 |
Database Link | |
Background | MASPIN is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. |
Synonyms | Serpin B5, PI5, PI-5 |
Note | Centrifuge vial prior to opening. |
Reference Data |
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