Kanamycin Sheep Polyclonal Antibody
CAT#: AP09735PU-N
Kanamycin sheep polyclonal antibody, Ig Fraction
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CNY 11,175.00
货期*
7周
规格
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Specifications
Product Data | |
Applications | ELISA |
Recommend Dilution | ELISA: 0.312 μg/ml. |
Host | Sheep |
Clonality | Polyclonal |
Immunogen | Kanamycin-BTG |
Specificity | This antibody recognizes Kanamycin. |
Formulation | 20mM Phosphate, 150mM Sodium Chloride State: Ig Fraction State: Liquid Ig fraction prepared by Caprylic Acid and Ammonium Sulphate precipitation procedures Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage Condition | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Background | Kanamycin (also known as kanamycin A) is an aminoglycoside bacteriocidal antibiotic, available in oral, intravenous, and intramuscular forms, and used to treat a wide variety of infections. Kanamycin is isolated from the bacterium Streptomyces kanamyceticus and its most commonly used form is kanamycin sulfate. Kanamycin is used in molecular biology as a selective agent most commonly to isolate bacteria (e.g., E. coli) which have taken up genes (e.g., of plasmids) coupled to a gene coding for kanamycin resistance (primarily Neomycin phosphotransferase II [NPT II/Neo]). Bacteria that have been transformed with a plasmid containing the kanamycin resistance gene are plated on kanamycin (50-100 ug/ml) containing agar plates or are grown in media containing kanamycin (50-100 ug/ml). Only the bacteria that have successfully taken up the kanamycin resistance gene become resistant and will grow under these conditions. As a powder kanamycin is white to off-white and is soluble in water (50 mg/ml). Mammalian cells and other eukaryotes are screened using G418, a similar aminoglycoside antibiotic, which KanMX confers resistance against. At least one such gene, Atwbc19 is native to a plant species, of comparatively large size and its coded protein acts in a manner which decreases the possibility of horizontal gene transfer from the plant to bacteria; it may be incapable of giving resistance to kanamycin to bacteria even if gene transfer occurs. |
Synonyms | Kanamycin A, Kantrex |
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