Cyclin B1 (CCNB1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunohistochemistry on Paraffin Sections: 1/50-1/100. Immunofluorescence: 1/100-1/200. Western Blot: 1/500-1/1000.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide sequence around aa.145~149 (A-F-S-D-V) derived from Human Cyclin B1
Specificity	This Antibody detects endogenous levels of total Cyclin B1 protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography using epitope-specific peptide
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	60 kDa
Gene Name	cyclin B1
Database Link	Entrez Gene 891 Human UniProt ID P14635
Background	In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at amino acids 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids 14Thr and 15Tyr of p34 cdc2, rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. Two alternative transcripts have been found, a constitutively expressed transcript, and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Synonyms	CCNB1, CCNB, Cyclin-B1
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Cell cycle, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation

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