Lep Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1/5,000-1/20,000. Western Blot: 1/500-1/2,000.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Mouse leptin 16,000 MW produced in E. coli.
Specificity	The antibody will recognize recombinant and native 16 kDa leptin from Mouse and Human. Reactivity with leptin from other sources is unknown.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative. State: Ig Fraction State: Liquid (sterile filtered) purified IgG fraction.
Concentration	lot specific
Purification	Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C prior to opening. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Gene Name	leptin
Database Link	Entrez Gene 16846 Mouse UniProt ID P41160
Background	Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.
Synonyms	LEP, OB, OBS, Obesity factor, Obese protein
Reference Data

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