Protein A Goat Polyclonal Antibody

CAT#: AP09120BT-N

Protein A goat polyclonal antibody, Biotin



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CNY 40,481.00


货期*
4周

规格
    • 10 mg

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Specifications

Product Data
Applications ELISA, IHC, WB
Recommend Dilution Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
The product has been assayed against 1.0 µg of Protein A in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Host Goat
Clonality Polyclonal
Immunogen Protein A [Staphylococcus aureus]
Specificity This antibody detects Protein A. Assay by immunoelectrophoresis resulted in a single precipitin
arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus]. Cross reactivity against Protein A from other sources is unknown.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 as buffer, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer, 0.01% (w/v) Sodium Azide as preservative
Label: Biotin
State: Lyophilized Ig fraction
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio: 10-20 BAC molecules per Goat IgG molecule
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Concentration lot specific
Purification Purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation Biotin
Storage Condition Store vial at 2-8° C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for two weeks at 2-8° C as an undiluted liquid. Dilute only prior to immediate use.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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