Horseradish Peroxidase / HRP Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western Blot. Dot Blot. Immunoprecipitation. ELISA: 1/9,000-1/40,000. This product has been assayed against 1.0 µug of Peroxidase [Horseradish] in a standard sandwich ELISA using Alkaline Phosphatase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and pNPP as a substrate for 30 minutes at room temperature.
Host	Goat
Clonality	Polyclonal
Immunogen	Peroxidase [Horseradish]
Specificity	This antibody recognizes Horseradish Peroxidase.
Formulation	0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 State: Serum State: Lyophilized
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Reference Data

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