PAG (PAG1) (97-432) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, IP, WB
Recommend Dilution	Immunohistochemistry on Paraffin Sections: 10 µg/ml. Immunoprecipitation. Western Blot.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG)
Specificity	This antibody recognizes Csk-binding protein (Cbp / PAG), a 46 kD ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kD molecule.
Formulation	PBS, 15 mM sodium azide, approx., pH 7.4 State: Aff - Purified State: Liquid purified Ig
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	phosphoprotein membrane anchor with glycosphingolipid microdomains 1
Database Link	Entrez Gene 55824 Human UniProt ID Q9NWQ8
Background	Csk binding protein, also known as PAG (phosphoprotein associated with GEMs), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Synonyms	PAG, CBP
Reference Data

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