Iba1 (AIF1) (149-161) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	IHC IHC-P (5 µg/ml) Peptide-ELISA (1:128000) WB (0.3 - 1 µg/ml)
Reactivity	Mouse, Rat, Hamster, Pig, Human, Monkey
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-TGPPAKKAISELP, from the C-Terminus of protein sequence according to NP_116573.1NP_001614.3.
Specificity	Human AIF1 / IBA1. This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
Formulation	TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene Name	allograft inflammatory factor 1
Database Link	Entrez Gene 199 Human UniProt ID P55008
Background	IBA1 antibody AP08912PU-N is an unconjugated goat polyclonal antibody to IBA1 (AIF1) (aa149-161) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Synonyms	AIF1, AIF-1, Em:AF129756.17, G1, IRT-1, Protein G1, IBA1, IRT1
Note	Immunohistochemistry: LS-B2645 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2645 was determined to be 5 ug/ml.
Reference Data

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