Ubiquitin (UBB) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 using streptavidin~biotin system or polymer system. FFPE tissue sections require antigen retriever, boiling tissue for 15-20 minutes in 10 mM citrate, pH 6.0, followed by cooling at RT for 15-20 minutes. Immunofluorescence. Recommended Positive Control: Human brain from Alzheimer's disease.
Reactivity	Bovine, Equine, Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified Human Ubiquitin.
Specificity	This antibody stains senile plaques and neurons. Cellular Localization: Nuclear, Cytoplasmic, and cell membrane.
Formulation	PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ubiquitin B
Database Link	Entrez Gene 7314 Human UniProt ID P0CG47
Background	Ubiquitin is a 76 amino acids 8.5 kDa, a highly conserved small regulatory protein that is ubiquitously expressed in eukaryotes and plays a central role in protein degradation. The Ubiquitin/proteosome pathways are main, non-lysomal route for intracellular protein degradation. The protein to be degraded is conjugated to Ubiquitin; it is selectively degraded by 26S complex multicatalytic cytosolic and nuclear protease termed proteosome. This protein also plays important role in cellular processes e.g. Cell cycle progression, gene transcription, antigen processing and signal transduction. In Immunohistochemistry (IHC) this antibody can detect abnormal inclusion bodies, in neurofibrillary tangles in Alzheimer’s disease, lewy bodies in Parkinson’s disease, pick bodies in Pick’s disease, inclusions in motor neuron disease, Mallory’s Haylin in alcoholic liver disease and Rosenthal fibers in astrocytes.
Synonyms	UBB, Polyubiquitin-B
Reference Data

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