Fibronectin (FN1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Immunohistochemistry: 1/100-1/200 for 30 minute at room temp. using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears require antigen retriever, boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temp. for 20 minutes. Positive control: Human kidney.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified human Fibronectin from human plasma.
Specificity	This antibody reacts with Human Fibronectin in liver, tonsil, skin and kidney. Cellular localization: Connective tissue matrix and basement membrane.
Formulation	10 mM PO4, 150 mM NaCl, pH 7.4 State: Serum State: Liquid purified Ig fraction Stabilizer: 1% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	fibronectin 1
Database Link	Entrez Gene 2335 Human UniProt ID P02751
Background	Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and extracellular matrix. Fibronectin is involved in cell adhesion and migration process including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They also occur as dimmer of two 200 kDa subunits. Fibronectin has been implicated in carcinoma development in lung cancer. Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents.
Synonyms	FN1, Cold-insoluble globulin, CIG
Reference Data

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