NFKB1 pSer927 Rabbit Polyclonal Antibody

CAT#: AP08022PU-S

NFKB1 pSer927 rabbit polyclonal antibody, Aff - Purified

Size: 50 ul 100 ul



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CNY 1,400.00


货期*
2周

规格
    • 50 ul

Product images

Specifications

Product Data
Applications IF, IHC, WB
Recommend Dilution Western Blot: 1/500-1/1,000.
Immunofluorescence: 1/100-1/200.
Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NFkB-p105/p50 around the phosphorylation site of Serine 927 (C-D-Sp-G-V).
Specificity This antibody detects endogenous levels of NFkB-p105/p50 only when phosphorylated at Serine 927.
Formulation PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State: Aff - Purified
State: Liquid purified Ig fraction.
Concentration lot specific
Purification Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Predicted Protein Size 120 kd
Gene Name nuclear factor kappa B subunit 1
Background NFkB is a transcription regulator that is activated by various intra and extra cellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Serine 529 is located in a negatively charged region (amino acids 422-540) that is phosphorylated in response to phorbol myristate acetate plus calcium ionophore activation. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth.
Synonyms NFKB1, KBF1, EBP-1, EBP1, NF-kappa-B p50
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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