Aurora A (AURKA) (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Immunoprecipitation. Western Blot: 0.5 - 2 µg/ml.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide from the C-terminus of human AURKA / Aurora-A (NP_003591.2)
Specificity	Recognizes C-terminus of Human AURKA / Aurora-A. Reported variants represent identical protein: NP_003591.2; NP_940835.1; NP_940836.1; NP_940837.1; NP_940838.1; NP_940839.1
Formulation	Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	aurora kinase A
Database Link	Entrez Gene 6790 Human UniProt ID O14965
Background	ARK1, an Aurora/IPL1-like protein kinase, functions in centrosome separation, duplication, and maturation and in bipolar spindle assembly and stability. The protein has been shown to localize to centrosomes in S phase as soon as centrioles have been duplicated, and to be degraded in early G1. ARK1 phosphorylation is induced by okadaic acid treatment in vivo. Activated ARK1 has been shown to phosphorylate the spindle apparatus-associated protein, TPX2, and it is believed to be targeted to the spindle apparatus by this protein. At least three alternative splice forms have been reported; however, the alternative splice sites are located in the 5’-untranslated region and appear to have no effect on protein translation. ARK1 has been identified as an oncogene that is overexpressed in several types of solid tumors and tumor cell lines, including those from breast and colon. Additionally, overexpression of ARK1 has been implicated in mechanisms leading to mitotic spindle aberrations, aneuploidy, and genomic instability.
Synonyms	AURKA, AIK1, ARK1, AURA, BTAK, STK15, STK6, Aurora/IPL1-related kinase 1, AURORA2
Reference Data

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