MDM2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Western Blot: 0.5 - 1 µg/ml.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide
Specificity	This antibody reacts to a synthetic peptide (gavecfeeedsnsresls) corresponding to amino acids 122 to 139 of MTBP, which differ from the mouse sequence by three amino acids.
Formulation	Phosphate buffered saline, containing 0.02% sodium azide State: Aff - Purified State: Liquid purified Ig
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	MDM2 proto-oncogene
Database Link	Entrez Gene 4193 Human UniProt ID Q00987
Background	The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, and MTBP, were recently discovered. The transcriptional activity of p53 is modulated by posttranslational regulations of the p53 protein including stabilization and acetylation. P53 transcriptionally activates MDM2 gene then the translated MDM2 protein binds to p53 and promotes the degradation of p53 leading to lowering the concentration of p53 protein. MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A recently discovered protein termed MTBP was found to bind to MDM2 and to inhibit the modulation effect of MDM2 on p53. MTBP is expressed in a variety of normal tissues.
Synonyms	p53-binding protein Mdm2, Oncoprotein Mdm2, Double minute 2 protein, Hdm2
Reference Data

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