p35 (CDK5R1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry on paraffin sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 1-54 of Human p35.
Specificity	This antibody detects endogenous levels of CDK5R1-p35 protein and does not cross-react with CDK5R1-p25 protein. (region surrounding Gln31)
Formulation	Phosphate buffered saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~35 kDa
Gene Name	cyclin-dependent kinase 5 regulatory subunit 1
Database Link	Entrez Gene 12569 MouseEntrez Gene 116671 RatEntrez Gene 8851 Human UniProt ID Q15078
Background	Cyclin dependent kinase-5 (Cdk5), a key regulator of cell cycle progression, was originally isolated on the basis of its structural homology to Cdc2, a wellcharacterized regulator of cell cycle progression. Although Cdk5 is expressed at the highest level in the brain of adult mice, intermediate levels in testis and low or undetectable levels in all other tissues, brain is the only tissue from which Cdk5 can be isolated as an active kinase. These findings may be explained by the cloning and characterization of a Cdk5 regulatory subunit, designated p35. p35 displays a neuronal cell-specific pattern of expression, physically associates with Cdk5 and activates Cdk5 enzymatic activity. p35 is also expressed in many tissues in a truncated form, designated p25.
Synonyms	CDK5P35; CDK5R; MGC33831; NCK5A; p23; p25; p35; p35nck5a
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease

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