SMAD4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200.  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 12-64 of Human Smad4.
Specificity	This antibody detects endogenous levels of Smad4 protein. (region surrounding Leu43)
Formulation	Phosphate buffered saline, pH 7.2 State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 60 kDa
Gene Name	SMAD family member 4
Database Link	Entrez Gene 4089 Human UniProt ID Q13485
Background	Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.
Synonyms	SMAD family member 4, SMAD-4, SMAD 4, MADH4, MAD homolog 4, DPC4
Reference Data

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