Caspase 3 (CASP3) Rabbit Polyclonal Antibody

CAT#: AP06504PU-N

Caspase 3 (CASP3) rabbit polyclonal antibody, Aff - Purified



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CNY 4,640.00


货期*
2周

规格
    • 100 ug

Product images

Specifications

Product Data
Applications IF, IHC, WB
Recommend Dilution Western blot: 1/500-1/1000.
Immunofluorescence: 1/50-1/200. 
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide, corresponding to amino acids 120-170 of Human Caspase 3.
Specificity This antibody detects endogenous levels of Caspase 3 protein.
(region surrounding Asp146)
Formulation Phosphate buffered saline (PBS), pH 7.2, 50% Glycerol
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Preservative: 0.02% mM Sodium Azide
Concentration 1.0 mg/ml
Purification Affinity Chromatography using epitope-specific immunogen
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Predicted Protein Size ~ 35.0 kDa
Gene Name caspase 3
Background The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10).
The processed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein.
In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells).Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Synonyms CASP-3, CASP3, CPP32, CPP-32, Yama protein, Apopain, SCA-1, SCA1
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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