Vimentin (VIM) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 411-460 of Human Vimentin.
Specificity	This antibody detects endogenous levels of Vimentin protein. (region surrounding Ile444)
Formulation	Phosphate buffered saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (>95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~48.0 kDa
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Background	Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer due to deficiencies in the DNA repair enzymes. The most frequent mutations are found in the XP genes of group A through G and group V, which encode nucleotide excision repair proteins. Nucleotide excision repair (NER) is the normal cellular response to DNA damage induced by UV irradiation and is disrupted in patients with XP. Xeroderma pigmentosum group A (XPA) is an essential NER factor that coordinates the collection of a preincision complex during the processing of DNA damage. XPA may also have a role in the repair of oxidized DNA bases. XPA is sensitive not only to the structure of the DNA double helix, but also to bulky groups incorporated into DNA. XPA forms a homodimer in the absence of DNA, but binds to DNA in both monomeric and dimeric forms. The dimerically bound XPA is much more efficient, so cells probably regulate XPA activity in a concentration-dependent manner. XPA deficient organisms cannot repair UV-induced DNA damage and thus acquire skin cancers by UV irradiation very easily.
Synonyms	VIM
Reference Data

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