USF2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 196-244 of Human USF2.
Specificity	This antibody detects endogenous levels of USF2 protein. (region surrounding Asp226/159)
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction Preservative: 15 mM sodium azide
Concentration	1.0 mg/ml
Purification	Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 36 kDa
Gene Name	upstream transcription factor 2, c-fos interacting
Database Link	Entrez Gene 7392 Human UniProt ID Q15853
Background	The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of 43 kDa (USF-1) and 44 kDa (USF-2) polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an upstream stimulatory factor that binds the core sequence CACGTG in the adenovirus late promoter. These findings, together with the demonstration of cooperative interaction between USF and the initiator-binding protein TFII-I, raise the possibility of a more general involvement of USF in transcriptional regulation. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and hetero-dimers are found in different cell types.
Synonyms	BHLHB12, FIP
Reference Data

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