RAF1 Rabbit Polyclonal Antibody

CAT#: AP06299PU-S

RAF1 rabbit polyclonal antibody, Aff - Purified

Size: 100 ug 25 ul



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CNY 800.00


货期*
2周

规格
    • 25 ul

Product images

Specifications

Product Data
Applications IHC, WB
Recommend Dilution Western blot: 1/500-1/1000.
Immunohistochemistry on paraffin sections: 1/50-1/200.
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide, corresponding to amino acids 211-260 of Human Raf-1.
Specificity This antibody detects endogenous levels of Raf1 protein.
(region surrounding Gln253)
Formulation Phosphate buffered saline (PBS), pH 7.2.
State: Aff - Purified
State: Liquid purified Ig fraction
Preservative: 0.05% sodium azide
Concentration 1.0 mg/ml
Purification Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Predicted Protein Size ~ 73 kDa
Gene Name Raf-1 proto-oncogene, serine/threonine kinase
Background Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.
Synonyms C-RAF, Raf-1, cRaf
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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