ASK1 (MAP3K5) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin sections: 1/50-1/200.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 924-978 of Human ASK1.
Specificity	This antibody detects endogenous levels of MEKK5 / ASK1 protein (region surrounding Glu960).
Formulation	Phosphate buffered saline (PBS), pH 7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). Preservative: 0.05% Sodium azide
Concentration	1.0 mg/ml
Purification	Affinity chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~155 kDa
Gene Name	mitogen-activated protein kinase kinase kinase 5
Database Link	Entrez Gene 4217 Human UniProt ID Q99683
Background	Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also called MAP kinase kinase kinases) phosphorylate and activate the MAP kinases, including ERK, JNK and p38. The MEK kinases characterized to date include Raf-1, Raf-B, MOS, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (also designated MEK kinase-5). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.
Synonyms	MAPK/ERK kinase kinase 5, MAPKKK5, MAP3K5
Reference Data

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