NF-kB p65 (RELA) pSer536 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/500-1/3000. Western Blot: 1/100-1/1000. Detects a band of approximately 65kDa in the appropriate cell lysate. For the detection of phosphoproteins, threonine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. Immunohistochemistry on Paraffin Sections: 1/50-1/250.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to residues surrounding serine 536 of Human NFkB p65 protein.
Specificity	This antibody detects the p65 (RelA) subunit of nuclear factor kappa-B (NFkappaB) when phosphorylated at serine 536.
Formulation	PBS, pH 7.2 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	RELA proto-oncogene, NF-kB subunit
Database Link	Entrez Gene 5970 Human UniProt ID Q04206
Background	NFkappaB is a heterodimeric DNA binding protein which was originally identified consisting of p65 and p50 (NF?B1) subunits. Research has led to the discovery of other subunits, including p52 (NFkappaB2), cRel, and RelB. NFkappaB is ubiquitously expressed, and functions as a second messenger which upon activation leads to the transcription of a number of genes. NFkappaB is activated by a number of agents, including pro-inflammatory cytokines and bacterial lipopolysaccharide, and is essential for the maturation of T cells and regulation of their survival and activation. Phosphorylation of serine 536 contributes to NFkappaB transactivation and increases p65 transcription activity.
Synonyms	NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3
Reference Data

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