FITC Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	ELISA: 1/4000-1/8000.
Host	Sheep
Clonality	Polyclonal
Immunogen	Fluorescein isothiocyanate (Isomer 1).
Specificity	This antibody recognises the fluorochrome Fluorescein Isothiocyanate (FITC) and has been cross-absorbed to minimise cross-reactivity with Mouse, Human and Rat serum proteins. This reagent may be useful for amplification of staining using FITC conjugated reagents.
Formulation	Phosphate Buffered Saline containing 50% Glycerol. Label: HRP State: Liquid purified IgG fraction. Label: Horseradish Peroxidase
Purification	Affinity Chromatography.
Conjugation	HRP
Storage Condition	Store the antibody undiluted at -20°C. Avoid repeated freezing and thawing.
Background	FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
Synonyms	Fluorescein Isothiocyanate
Reference Data

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