EDG6 (S1PR4) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western Blot: Detects EDG-6 receptors at concentration of 5-10 µg/ml by Western blot using a Human EDG-6 receptor transfected cell line. Detects an approximately 45 kDa band in Cos cells transfected with full length Human EDG-6. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development. Positive Control: RH7777 cells transfected with EDG6 protein.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide derived from the C-terminal of the EDG-6 receptor.
Specificity	This antibody recognizes Human EDG-6 / S1P4 / S1PR4. Other species not tested.
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.08% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulfate Precipitation
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	sphingosine-1-phosphate receptor 4
Database Link	Entrez Gene 8698 Human UniProt ID O95977
Background	EDG-6 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-6 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-6 is expressed in lymphoid and hematopoietic tissue and in the lungs. Recently, the designation of the EDG receptors has been modified to include the ligand for the receptor. Thus, the new designation for the EDG-6 receptor is the sphingosine 1-phosphate receptor-4 (S1P-4).
Synonyms	EDG-6
Reference Data

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