Adenovirus E1A (E1A) Sheep Polyclonal Antibody

Specifications

Product Data
Applications	IP, WB
Recommend Dilution	Western Blot: 1-10 µg /ml. Positive Control: 293 cells are human embryonic kidney cells that have been transformed with the adenovirus genes E1A and E1B, which are required for adenovirus propagation. This cell line can be used as a positive control for this antibody.
Reactivity	Adeno-Associated Virus
Host	Sheep
Clonality	Polyclonal
Immunogen	GST-EIA fusion protein recognizing a 43kD protein.
Specificity	This antibody reacts to Adenovirus E1A.
Formulation	Phosphate buffered saline with 0.08% Sodium Azide State: Purified State: Liquid purified Ig (0.2µm sterile filtered)
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Background	The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105. EIA gene encodes potent onco-proteins that modify the normal transcriptional growth regulation of key cellular genes and thus alter cell cycle control.
Synonyms	Adenovirus early region 1 antibody, Control protein E1A antibody, E1A antibody
Reference Data

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