p73 (TP73) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western Blot: 1:500~1:1000.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized non-phosphopeptide derived from human p73 around the phosphorylation site of tyrosine 99 (S-P-YP-A-Q).
Specificity	p73 antibody detects endogenous levels of total p73 protein.
Formulation	PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity-chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	tumor protein p73
Database Link	Entrez Gene 7161 Human UniProt ID O15350
Background	p73 protein is a member of the p53 family of proteins. The tumor-suppressor protein p53 exhibits sequence specific DNA binding, directly interacts with various cellular and viral proteins, and induces cell cycle arrest in response to DNA damage. In response to signals generated by a variety of genotoxic stresses, e.g, UV irradiation or DNA damage, p53 is expressed and undergoes post translational modification that results in its accumulation in the nucleus. Activation of p53 leads to cell cycle arrest and in some cases to apoptosis, resulting in the inability of genetically damaged cells to proliferate. Thus, the p53 dependent pathways help to maintain genomic stability by eliminating damaged cells. The accumulation of high levels of p53 is a potential marker for malignancy. p73 protein is expressed in either full length form (p73 alpha 80 kDa) or a shorter (p73 beta 70 kDa) mRNA variant. p73 gene was predicted to encode a protein with significant amino acid sequence similarity to p53. Each of the p53 amino acid residues implicated in direct sequence specific DNA binding is conserved in p73. p73 can activate the transcription of p53 responsive genes and inhibit cell growth in a p53 like manner by inducing apoptosis and cell cycle arrest. p73 has been mapped at chromosome 1p36.3, which is a locus of multiple suppressor genes for tumors including neuroblastoma and other cancers. c-Abl stimulates p73 mediated transactivation and apoptosis. This regulation of p73 by c-Abl in response to DNA damage was also demonstrated by a failure of ionizing radiation induced apoptosis after disruption of c-Abl-p73 interaction. The p73 protein can activate p53 responsive promoters and induce apoptosis in cells deficient in p53, which proves that inactivation of p53 family members may contribute to the biological properties of a subset of p53 mutants. The transcriptional activities of p53, p73 alpha, and p73 beta to induce their common cellular target genes differ among one another. Despite the high degree of similarity between p53 and p73, adenovirus E1B 55K, simian virus 40 T, and human papilloma virus E6 do not physically interact with p73, indicating that the functions of these two proteins may differ.
Synonyms	OTTHUMP00000003479; OTTHUMP00000003480; P73
Reference Data

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