PDPK1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/500 - 1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry on paraffin sections: 1/50 - 1/100. Immunofluorescence: 1/100 - 1/200.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic non-phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241(A-N-SP-F-V).
Specificity	PDK1 antibody detects endogenous levels of total PDK1 protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer. State: Aff - Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	3-phosphoinositide dependent protein kinase 1
Database Link	Entrez Gene 5170 Human UniProt ID O15530
Background	Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
Synonyms	PDK-1, PDK1, 3-phosphoinositide-dependent proteinkinase 1, hPDK1, PDPK1
Reference Data

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